What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,041 4 View

How do I reduce the size of a file in Comsol simulations?

I am working with RF module in comsol and doing parametric sweeps in frequency domain. The data generated is huge and taking a lot of space on my disc. The fine meshing that I need for my problem...

22 July 2020 4,374 6 View

How to get the antenna to resonate at the required frequency with the ideal magnitude in the s-parameter graph?

I have designed a rectangular patch antenna for a frequency of 2.4Ghz using Rogers 5880 as my substrate material with a thickness of 1.6mm. But, my s-parameter graph does not show a graph with the...

03 October 2019 6,543 4 View

Best reagent for Colorimetric detection of chloride ions?

I want to find an assay for colorimetric method of detection of chloride ions from blood or body fluids. The main issue is I cannot use mercury or mercury derived chemicals in my assay. Can...

04 February 2019 8,093 3 View

How to grow N.gonnorhea?

02 August 2016 1,264 1 View

I tried extracting plasmid DNA by Brimbroim Dolly method. After running on agarose gel I am getting mutiple bands of different molecular weights.why?

Are all the bands plasmids itself?or is it degraded plasmid?

30 July 2016 7,069 11 View

Can you provide any method for APEO analysis on HPLC?

bufffer of what pH can we use as mobile phase?

01 March 2016 8,861 1 View

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

11 February 2016 1,768 2 View

What is the difference in FWHM from Guassian and Lorent fit used in Scherrer formulae?

I have calculated the crystallite size of NiO by using the well known Scherrer formulae. I fit the XRD peaks and determined the FWHM both by Gaussian and Lorentz fit however the crystallite size...

14 June 2015 770 27 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View