The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

How to examine the substitution of ligands bound to protein during the MD simulation?

I have simulated a complex ( A protein docked with two ligands), and the ligands are bind to two different protein sites. Still, from the start of the simulation, ligands are positioned in other...

27 November 2020 7,848 4 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

Is it possible that formazan Crystal formation took more than four hours ?

Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...

20 October 2020 1,106 3 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

How to increase the number of nanoparticles in fe-sem?

I produce a protein nanoparticle complex,but in fe-sem, I see a few number of nanoparticles,maybe 2 or 3 ,however the color of suspension solution is quite milky and this is a sign of...

29 August 2020 8,812 5 View

Why would a negative control have apoptosis in the Annexin V-PI Flow Cytometry?

06 August 2020 3,627 22 View

How can we induce gastroesophageal reflux disease in rats?

Gastroesophageal reflux disease (GERD) occurs when stomach acid frequently flows back into the tube connecting your mouth and stomach (esophagus). This backwash (acid reflux) can irritate the...

14 June 2020 145 8 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

How can i perform logistic regression on nominal vs scale variables in SPSS?

Dear all, I would like to perform a logistic regression analysis on the correlation between surgery duration time represented in minutes (Scale independent variable) with rate of neuropraxia as...

14 February 2021 4,054 3 View

Is it reliable to evaluate surface roughness (gwyddion) with SEM pictures?

There are a few works(e.g. DOI 10.1002/sia.4889) reporting that surface roughness can be evaluated by software like gwyddion with SEM pictures.However,the surface roughness is strongly influenced...

09 February 2021 1,905 8 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

What are the options for statistical analysis to find the relation between nominal, interval and ordinal data ?

I have 7 variables (1 interval, 4 ordinal and 2 nominal) with levels between 4-9, for which responses are received through a survey. My question is how I can find the relation between these...

27 January 2021 8,805 6 View

Which statistical test is suitable to test if 2 explanatory variables (different scales: ordinal & categorical) predict dependent variable (ordinal)?

I'm working on survey data that is inclusive of ordinal (1-5 rating scale), categorical (yes/no), nominal (frequency response). I have reduced many ordinal variables (those in ordinal scale) to 3...

26 January 2021 804 12 View

Among all the environmental pollutants globally how much % out of 100% is due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world is suffering. Among all the environmental pollutants and hazardous materials how much contribution is from...

31 December 2020 6,039 3 View

Among All the environmental pollutants how much % out of 100% are due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world. Among all the environmental pollutants and hazardous materials how much contribution is from chemists (out...

31 December 2020 833 7 View

How do you describe the effect of surface roughness in 3 point bend simulation in Abaqus?

Hello everyone, Part of my project is to investigate the effect of surface roughness on stainless steel 17-4 PH in a 3-point bend simulation on Abaqus. I was to use 2D planar mode and manually...

21 December 2020 5,675 3 View