I wanted to investigate about apoptosis in mouse oocytes, but it's a new field to me (both apoptosis and mouse oocytes- I only worked with human cell lines before), so I hesitated to choose the good options to check apoptosis in mouse oocytes. I saw people often use COMET assay, TUNEL assay, Anexin V (flow cytometry or immnufluorescence), Western blot with PARP or casp 3 cleavage in different studies. Are there any advantages or disadvantages of those to use in mouse oocytes? (From what I see Anexin V is used on non-fixed cells, while COMET/TUNEL can be on fixed cellsbutI'm not sure for oocytes if it's better to go with fixed or non-fixed methos. And Western blot and Flow cytometry might be hard since I need to have a high number of oocytes). 

Thank you.