Phuong Nguyen

4 Questions 4 Answers 0 Followers

Questions related from Phuong Nguyen

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

13 January 2021 696 1 View

What can be the good method to check apoptosis in mouse oocytes?

I wanted to investigate about apoptosis in mouse oocytes, but it's a new field to me (both apoptosis and mouse oocytes- I only worked with human cell lines before), so I hesitated to choose the...

20 September 2017 2,953 2 View

The basic way to calculate UV dose from a UVC 15Watt bulb?

I plan to use a UV lamp to create DNA breaks in the cells. The UV lamp in our lab is fixed in the cell culture hood (Ushio germicidal bulb, UVC, wavelength 253.7 nm, 15 watt, 55 volt) and I need...

12 March 2017 7,334 19 View

Double strand break assay: Ionizing radiation or ETOP treatment for lymphocyte cells?

I am planning to perform double strand break (DSB) assay to test how cells response to DSB (patients vs controls) (general design: induce DSB and measure the level of γ-H2AX foci). I could see...

12 September 2016 3,327 7 View