What buffer should I add to the Krebs-Henseleit solution to counteract the rise of the PH while using pure oxygen instead of carbogen?

More Angélica María Estrada's questions See All

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Can the content of my research section be shown on the front page?

no details needed

26 December 2020 9,690 3 View

Has anyone used ANSYS Student Software to model light propagation?

I'm wondering if one is able to model light propagation in an optical setup using ANSYS Student Software or if it is only possible to do so using SPEOS, which to my knowledge is not included in...

20 February 2020 1,184 1 View

How do you determine Band Gap for a solid, opaque material?

I have seen many answers to the question on how to determine the band gap of a semi-conductor, but that always implies the use of UV-Vis Spectroscopy. However, the problem with this method is that...

28 January 2020 8,981 7 View

How to be more productive while writing?

Do you have any specific schedule when is the best time to write a publication, an article for a journal or an academic book? When is the best productive time for it? Please, share your experiences

29 December 2019 1,705 4 View

In dissipation engineering. Why the microscopic mechanisms of damping are limited by low frequencies?

I am starting working on damping in metamaterials, and I found the following statement that caught my attention: "Dissipation engineering at the macroscopic level is additive; it builds on what...

05 June 2019 9,566 4 View

How can I improve a DNA extraction from serum?

Hi everyone! I'm working on a DNA extraction from serum. Unfortunately the concentration was very low and the PCR amplification didn't work, maybe because the DNA extracted was very fragmented. Do...

23 August 2018 4,155 3 View

How can I improve a DNA extraction from serum?

Hi everyone! I'm working on a DNA extraction from serum. Unfortunately the concentration was very low and the PCR amplification didn't work, maybe because the DNA extracted was very fragmented. Do...

07 August 2018 518 4 View

What is the most cost and time effective way to assess the isoprene synthesis capacity of a poplar leaf?

Dimethyl allyl diphosphate (DMADP) is transformed to isoprene and pyrophosphate (PPi) thanks to the isoprene synthase. Is it better to: - measure the concentration of isoprene synthase assuming...

05 June 2018 3,208 2 View

Is it enough an Exonuclease treatment before sequencing?

Hi everyone! I would like to know if an exonuclease I treatment after isolating DNA samples from an agarose gel is enough for sequencing. I know that it is recommended to use ExoSap which...

04 May 2018 5,413 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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24 February 2021 5,749 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Malcolm Caulfield

We used to use 5mM HEPES

Charles Michael Pearman

HEPES at concentrations between 5 and 20mM is commonly used as a pH buffer during Langendorff-perfused heart preparations, an alternative is MOPS.

For a review article (mainly about cell isolation but also containing a section on physiological solutions and the rationale behind their constituents) see Article Methods for isolating atrial cells from large mammals and humans