What bone biomarkers (proteins) to be used to evaluate bone regeneration in vitro using western blotting?

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Yahia Mehdi Seddik Cherifi

Hi Heba,

There are a lot of them and some depend about the research field you work.

The better for you is to read these articles:

Donatella Granchi, Enrique Gómez-Barrena, Markus Rojewski, et al., “Changes of Bone Turnover Markers in Long Bone Nonunions Treated with a Regenerative Approach,” Stem Cells International, vol. 2017, Article ID 3674045, 11 pages, 2017. doi:10.1155/2017/3674045

J. Ma, S. K. Both, F. Yang et al., “Concise review: cell-based strategies in bone tissue engineering and regenerative medicine,” Stem Cells Translational Medicine, vol. 3, pp. 98–107, 2014.

D. J. Leeming, P. Alexandersen, M. A. Karsdal, P. Qvist, S. Schaller, and L. B. Tankó, “An update on biomarkers of bone turnover and their utility in biomedical research and clinical practice,” European Journal of Clinical Pharmacology, vol. 62, pp. 781–792, 2006.

I hope this would help you.

Saba Tariq

Dear Heba,

This article will help you.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427692/

Best wishes for your work

Regards

Dr Saba

Amin Shavandi

You may look for bands of FGF2 and BMP-2 proteins.