What blocking buffers can I use for a Lateral Flow Immunoassay?

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Why my colony PCR, after LR reaction not working?

I inserted my D-product artificial-miRNA into pDONR207 DH5alpha, colony confirmed with PrimerA , PrimerB also with attB1, and attB2 primers. similarly, the plasmid was confirmed with the same...

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Sequence Synthesis "T" Meaning ?

I want to synthesized one Probe from one publish article, but its showing like "T" in between the sequence? Can anyone knows what is the meaning for the same? [6FAM]...

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How to convert HIV Copies/mL to IU/mL ?

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Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

05 February 2019 358 10 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

02 December 2018 1,794 9 View

Can someone please suggest any open source Wireless Network Monitoring software ?

Hello Friends, I am looking for a open source software, which can allow me to monitor the parameters of a wireless network. 1. Serving Cell RSRP, RSRQ & SINR. 2. Neighbor Cells RSRP. 3....

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Which Gene Internal Control Best for DNA Real Time PCR?

I want to amplify DNA with Internal Control

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What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

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Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

06 October 2017 9,488 3 View

Can someone guide me through the procedure of a Von Neumann stability analysis for a 2-D Viscous Burgers equation?

I have used this technique for the 2-D heat equation but am not sure how to apply it to a system of equations (i.e. a vector PDE). Do the errors for the dependent variables have the same Fourier...

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What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

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Substitutes for Dako anti-HBcAg rabbit polyclonal?

I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...

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How do results of Bland-Altman plots and regression analysis contribute to our understanding of the comparability between serum tubes and LIH tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...

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Aside from the diagnostic efficacy of the various immunoassays for HDV, do these tests vary in risks or health hazard for the medical technologist?

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What are the factors that make lithium heparinized plasma (LIH) tubes more viable for use in clinical laboratories?

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08 April 2024 3,341 3 View

Is it economically advantageous to purify polyclonal antibodies for lateral flow immunoassays?

Dear researchers, I am interested in the purification process of polyclonal antibodies for use in lateral flow immunoassays. Specifically, I would like to inquire about the common practices and...

14 February 2024 9,120 2 View

Insulin unit conversion method ?

"Using the Insulin unit conversion method (1 µIU/mL = 0.04 ng/mL), can this be applied to measurements taken by ELISA (pg/mL) rather than ECLIA (Electrochemiluminescence Immunoassay)? This...

01 January 2024 7,504 1 View

What does the meaning of numbers that given in the Result of qualitative type of ELISA?

I am working with NOVATECH ELISA KIT for Brucella. In the leaflet, it is mentioned that it is a qualitative assay with a cutoff starting at 11. When I enrolled the assay through the DS2 automatic...

09 December 2023 6,166 3 View

How can the quantity of particles accumulating at the sample pad-nitrocellulose membrane interface in a lateral-flow immunoassay be reduced?

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16 November 2023 4,785 3 View

We have Bio-Rad iMark Microplate reader in our lab. How could we use this instrument in plant biology research? What biological assays could we can?

About application of Bio-Rad iMark Microplate reader beyond immunoassays.

13 July 2023 295 3 View