Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

05 February 2019 224 10 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

02 December 2018 1,662 9 View

Can someone please suggest any open source Wireless Network Monitoring software ?

Hello Friends, I am looking for a open source software, which can allow me to monitor the parameters of a wireless network. 1. Serving Cell RSRP, RSRQ & SINR. 2. Neighbor Cells RSRP. 3....

31 August 2018 6,474 6 View

Which Gene Internal Control Best for DNA Real Time PCR?

I want to amplify DNA with Internal Control

21 August 2018 399 3 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

10 April 2018 8,404 4 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

06 October 2017 9,370 3 View

How do I get the required value for modulation index "m" to obtain 1 pu generated voltage by the converter?

25 September 2015 977 4 View

How can I generate multiple triangular waveform (6 numbers of carrier waveform) each with a phase shift of 90 degree in MATLAB?

All the carrier should have same carrier frequency(say 1000Hz). I have used repeating sequence for the triangle waveform generation and it is working. Which simulink block can I use for a phase...

04 September 2015 9,426 6 View

How can one make a PWM controller (using chopper) for wound rotor induction motor?

How should the duty cycle be varied to vary effective external resistance and how can it be implemented?

08 March 2015 2,215 2 View

Can anyone Provide me the protocol for Real Time PCR for MicroRNA Amplification and detection?

07 December 2014 6,359 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View