Ram Gopal Nitharwal

6 Questions 28 Answers 0 Followers

Questions related from Ram Gopal Nitharwal

Proteins in novex-Thermo native page marker?

I am running a native gel for SOD assay. I have used the thermo/novex native page marker (Catalog number: LC0725) in the same gel. Strangely I am detecting 7 sod positive band between 66 kDa and...

08 August 2017 3,379 0 View

In vivo cross linking of mycobacterial membrane proteins?

I am trying to purify a membrane complex from mycobacteria. It seems that the interaction is very weak within the complex so I am not able to purify the whole complex. I am thinking to fix the...

09 September 2016 9,899 2 View

Why avidin is required for strep tag purification?

I am working with purification of protein by strep tag. In my protocol there is avidin in the binding buffer. Right now I do not have it. Can I proceed with the purification without adding avidin....

08 August 2016 1,170 3 View

How is GFP plus different compared to GFP?

I am working on tagging a protein with GFP plus. I need to know how it is different from tagging with GFP.

07 July 2016 3,123 2 View

The concentration range of histidine for elution of proteins bound to Ni-NTA.

I am using Ni-NTA method for protein purification. Due to buffer constraints I want to elute the protein with Histidine. Can anyone let me know what conc. of His I should include in my wash and...

07 July 2016 9,675 6 View

How to isolate the quinol pool from the membranes?

I am interested in isolation of total quinols from the membranes. I want to use it for in vitro assay of respiratory supercomplex3-4.

01 January 1970 3,405 2 View