6 Questions 28 Answers 0 Followers
Questions related from Ram Gopal Nitharwal
I am running a native gel for SOD assay. I have used the thermo/novex native page marker (Catalog number: LC0725) in the same gel. Strangely I am detecting 7 sod positive band between 66 kDa and...
08 August 2017 3,380 0 View
I am trying to purify a membrane complex from mycobacteria. It seems that the interaction is very weak within the complex so I am not able to purify the whole complex. I am thinking to fix the...
09 September 2016 9,900 2 View
I am working with purification of protein by strep tag. In my protocol there is avidin in the binding buffer. Right now I do not have it. Can I proceed with the purification without adding avidin....
08 August 2016 1,171 3 View
I am working on tagging a protein with GFP plus. I need to know how it is different from tagging with GFP.
07 July 2016 3,124 2 View
I am using Ni-NTA method for protein purification. Due to buffer constraints I want to elute the protein with Histidine. Can anyone let me know what conc. of His I should include in my wash and...
07 July 2016 9,677 6 View
I am interested in isolation of total quinols from the membranes. I want to use it for in vitro assay of respiratory supercomplex3-4.
01 January 1970 3,406 2 View