I would also be interested in a working protocol to treat water with DEPC (Concentration of DEPC to be used, autoclave temperature, drying procedures).
Marine environemnt faced lot of issues like pollution and biodiversity loss due to industrialization and other ocean related activities. We should think seriously about the marine conservation for...
29 May 2020 5,423 1 View
For desalination membrane technology is a common practice. Recently National Institute of Ocean Technology has successfully established Low Temperature Thermal desalination plant (LTTD) for the...
13 January 2015 9,050 6 View
I have done some work on rhythmic activity of some estuarine and marine fishes. This kind of research work will be more useful in aquaculture practices
11 January 2015 4,122 5 View
In India we have deployed Fish Aggregating Devices at Andaman & Nicobar Islands and Lakshadweep group of Islands for the benefit of Fishermen community as requested by the respective Fisheries...
17 December 2014 4,176 15 View
I am planning to extract RNA from a rice sample using a kit from Roche. Has anyone used it already and has feedback about the kit for isolating RNA from a rice sample?
13 November 2013 2,142 5 View
Suggestions for India’s Blue Economy This is regarding India’s Blue Economy Policy. We are happy to see lot detailed information on India’s Blue economy Policy. Definitely we can get good economy...
01 January 1970 9,231 2 View
There are literature available to predict the residual stress after machining in a single phase material by ABAQUS. . But how to predict it in a two phase material (duplex stainless steel)?
01 January 1970 8,993 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View