These are universal primers for 16S rRNA amplification;
F24 (5’- GAG TTT GAT YMT GGC TCA G -3’)
Y36 (5’-GAA GGA GGT GWT CCA DCC-3')
What are the representative pairs for YMWD?
R = A or G
Y = C or T
S = G or C
W = A or T
K = G or T
M = A or C
B = C or G or T
D = A or G or T
H = A or C or T
V = A or C or G
N = any base
Cheers, Nadine
Hi, look at this website:
http://www.bioinformatics.nl/molbi/SCLResources/sequence_notation.htm
Yeah But X is nowhere to be found..I thought it was an error..Any idea?? Thank you in advance .
Ocean, i have the same question.
I saw a primer sequence with X in it.
Anyone who can help out?
Dear Mohsen Sohrabi , really good question and these answers helped to solve my problem.
I think this website is useful
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwjkzu-xkZHxAhXoxIsKHZlZDuoQFjAMegQIDxAD&url=https%3A%2F%2Fwww.addgene.org%2Fprotocols%2Fprimer-design%2F&usg=AOvVaw3uJs8srxN-rATTM20p0buD
R = A or G.
Y = C or T.
S = G or C.
W = A or T.
K = G or T.
M = A or C.
B = C or G or T.
D = A or G or T.
ببخشید کمی دیر شد
Recognition sequences representations use the standard abbreviations to represent ambiguity:
R = G or A Y = C or T M = A or C K = G or T S = G or C W = A or T B = not A (C or G or T) D = not C (A or G or T) H = not G (A or C or T) V = not T (A or C or G) N = A or C or G or T
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