Donia Alson

3 Questions 6 Answers 0 Followers

Questions related from Donia Alson

Why is only kappa chain primers are used and not lambda chain primers in most of the studies to generate monoclonal antibody production?

I am trying to generate monoclonal antibodies (murine Fab, BALB/c mice) by the phage display method. Many studies focused on amplifying the kappa region of the light chain. Isn't that both kappa...

16 October 2020 9,220 4 View

Phage Display - How many different number of cDNA for the cDNA pool to be cloned into the phagemid vector to be transformed to bacteria?

I am trying to generate monoclonal antibody by phage display method. After mRNA to cDNA conversion, how many numbers of cDNA from the cDNA pool to be cloned into phagemid vector. What determines...

18 September 2020 9,445 4 View

How to study the function of two isoforms of a protein, when it is difficult to design a primer for both of them?

22 November 2016 3,988 3 View