Fungi spores

Thank you but I don't think it is Fungi spores

what is the prevalence on your slides and across your hosts? do you fix your slides in the field straight after collection?

Thank you Edward, it fixed in Pure ethanol. there are many of them in the slide

how have you take the blood sampl from lizards!!

Blood sample from limb

the best place that you can take blood  (look to attachet file), but I think that is small particul look like particule of celle!!!!!!

the best source that you can take is :HEMOPARASITES OF

THE REPTILIA Atlas (SAM ROUNTREE TELFORD, JR., 2009)

Thank you for your kind advice

Yes I agree Telford 2009 is the best source to go through, and taking blood from the caudal vein is probably the easiest. However, I dont think that is what is causing your "strange things" on your slide (unless the host reptile is being put under lots of stress?).

I dont think these are particles of cells, I think it could be some sort of virus or bacteria (I have found some of these, but only in the blood cells), have you tried sequencing any of your samples? It also could still be as J.C. Toriano suggested be a fungus, however this is unlikely (especially if you fixed them in ethanol directly after collection), and fungi spores usually look a bit different.

If I was you I would try sequencing them to see if you can pick anything up, and to see if you are getting the same results from other infected samples and nothing from samples not infected.

What is the percentage of slides positive to not infected?

Do you fix them and stain them the same day, or do you fix them in the field and stain them a few weeks later in the lab? I would use methanol to fix the slides instead of ethanol.

Make sure your reptiles are not under to much stress. Do you use traps, or do you collect by hand? If using traps how long are then left in the traps and how long do you keep them before taking the blood samples? 

Sorry I can not be of more help... 

Thank you all

hello I think Fungi spores

Fungi spores can be but thinking in accidental contamination with polen.