If a gene sequence is completely/nearly the same in different species, does that mean there is a gene homology?
To detect this homology and its meaning, you have to use program for multiple sequence alignments. See attached article for more information
Article Assessment of molecular (dis)similarity: The role of multipl...
Thanks for your interest. So we trying write perl codes with global alignment matrices but i find it hard.
If I understood right and if you aim to run MSA, then there is no need for Perl codes. You may use any open software. Clustal, Mega....
http://www.ebi.ac.uk/Tools/msa/.
Based on current global issues, the main threats at the international level can be summarized as follows: 1. Particularly, global pandemics can pose serious health threats on an international...
29 January 2024 313 4 View
Geopolitical factors influence intelligence operations in various ways: 1. Geopolitical considerations assist intelligence agencies in determining strategic priorities. The political, economic,...
29 January 2024 1,621 0 View
Do you have any experince to etch 20 nm Au with DRIE by using Ar or Cl2? Do you have any suggestion about thin gold etching? Note that as a etching mask, I will not use metal layer, just polymer mask.
28 January 2024 2,338 0 View
My data has multiple straight line regions for both anodic and cathodic reaction
05 November 2023 645 0 View
In my hypothesis driven SEM, I have in total 28 variables and 58 edges. Considering such amount, do you suggest me to report adjusted p-values with FDR for example or raw p-values? Thank you in...
15 August 2023 5,489 5 View
I am using N2 gas pump for continuous buffer flow in plastic microfluidics channel.
10 August 2023 8,903 1 View
The driver seems DNA based on the parallelisms below? (?a nonstop dominance struggle in DNA between progressive and retrogressive forces?) Evidence for the parallelism between extreme warmings...
09 June 2023 6,230 10 View
Hi everyone, I had a patient 3 days ago complaining of left ear fluctuating sensorineural hearing loss, otofonia before attacks, and vertigo(lasts one hour). his test results : unterberger has no...
13 May 2023 7,060 2 View
No, quantum entanglement does not allow for the violation of the speed of light limit. Quantum entanglement is a phenomenon in which two or more particles become correlated in such a way that the...
02 March 2023 6,936 3 View
Hi All, Normally when we are preparing the PDMS, we usually use Si as mold . Assume that you fabricate your PDMS mold in 3D-Printer instead of patterns on Si fabricated by SU-8 . Do you think...
27 January 2023 4,346 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View