It's not a secret that while truly pure imidazole does not have absorbance above 260 nm, typical imidazole products on the market (such as ACS, "Ultrapure", etc grades) do have absorbance of about 0.6-0.9 at 280 nm and 305 nm peaks.
What are the impurities which give these two absorbance peaks?
Dominique Liger
Hi there,
Before any measurement you should prepare your solutions in a buffer instead of water to work at a fixed pH. And check pH (and possibly adjust it) after dissolution.
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Edward Michelini
There are some purification methods covered in Armarego p 400 , but none appear easy, good luck. Google "continuing demand for the Purification of Laboratory Chemicals book" and there may be a copy online.
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