Ideas about drug-polymer microparticles characterization?

31 December 2016 9,567 8 View

What are the pharmaceutical propierties of luteolin?

Someone can suggest me some articles about uses of this drug. Thank you!

03 April 2015 3,977 6 View

How can I study the release of curcumin in PBS or in a medium to simulate an human tissue?

03 April 2015 9,234 9 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

02 March 2015 7,841 2 View

How can I determine the intensity of fluorescence of Fluorescein isothiocyanate?

I want to demonstrate that FITC is present in a powder obtained by the micronization method.

02 March 2015 1,222 3 View

I dissolved dextran in 25 mL of water and then added 25 mL of ethanol. why did a gel form? how can I avoid it?

I want to dissolve dextran in a mixture of water/Ethanol.

01 February 2015 3,466 8 View

Can I use curcuma used in kitchen for curcumin to encapsulate a model drug in a polymer?

31 December 2014 8,088 1 View

How can I use quantitative FT-IR?

31 December 2014 1,373 2 View

Are there articles about FTIR spectrum of FITC?

Read 2 answers by scientists with 1 recommendation from their colleagues to the question asked by Alessia Di Capua on Jan 3, 2015

31 December 2014 5,331 1 View

Which drug is interesting to be monitored by the fluorescein?

I need a drug which is soluble in water.

31 December 2014 1,659 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View