Why are there two reduction peaks in my cyclic voltammogram?

When I measured the cyclic voltammogram of palladium in the pbs solution, two reduction peaks appeared. This did not match the literature I saw. Why? And how can I find the possible reaction...

20 February 2021 2,486 3 View

Why am I unable to coat oleate-capped NaCeF4:Gd,Tb nanoparticles with mesoporous silica shell?

My oleate-capped NaCeF4:Gd,Tb NPs have been synthesized via the thermal decomposition method. 1mmol of NPs has been dispersed in 20 ml of cyclohexane. I have tried dissolving 1 ml of NP in...

01 December 2020 4,713 2 View

How to explain reason for using 0.1 as margin of error?

I currently conduct research on factors that influence career choice and I decided to use 0.1 as the margin of error. My supervisor asks me to explain the reason the value is selected. What reason...

23 November 2020 4,764 2 View

How to analyze correlation of multiple answer dependent variable with independent variable using SPSS?

My research had a multiple answer dependent variables. How can I use spss to find the correlation of the dependent variable and independent variables? Should I use define multiple response set first?

23 November 2020 753 3 View

How can I decrease surface roughness in selective laser melting?

Recently, I study master degree in Taiwan. And my research topic is about metal 3-d printing with in718. I am required to decrease surface roughness in overhanging structure or inclined cube. Is...

02 November 2020 8,127 3 View

Can we assume the water bottom reflectacne does not change and remain at a certain value?

Hi guys, I am trying to understand if the water bottom type does not change, its reflectance at a certain wavelength changes or not?

15 October 2020 6,297 3 View

How to gain the access to image recognition?

I am graduate from other major, and now working in computer science and technology, my roomates are mostly researching on image recognition. I want to switch to it and learn how to cooperate with...

21 August 2020 2,888 9 View

Final stage process development for lipid nanoparticles?

Dear Colleagues, To design a final stage process development for lipid nanoparticle formation using microfluidics, what aspects should be taken into account? Thank you for your...

09 August 2020 750 3 View

What are the current research results on est- SSR primers in azaleas?

I plan to conduct research on est-ssr primers of rhododendrons. I would like to know the research results that have been completed so far. If anyone can give me some Suggestions, I will be very...

31 May 2020 3,936 3 View

Why did I get so many negative clone after electronic shock when constructing a phage library?

I use phagemid pCDisplay3 and M13KO7 to construct Human Naive phage library. before digested with SfiI , I prepared ScFv (750bp) using gel purification. After electronic shock , ,plating on...

26 March 2020 4,023 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

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02 March 2021 932 1 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

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19 February 2021 8,647 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

May I know how to determine the acceptable intakes for new types of nitrosamine discovered in drug?

Currently only common nitrosaminse like NDMA and NDEA have mentioned the acceptable daily intake. Based on EMA guidance, should refer to ICH M7 chapter 7.5 guideline. I have read but could not...

20 January 2021 522 1 View

The First Survey Addressing Patients with BMIs over 50?

Dear Bariatric Surgeons, The importance of obesity management in patients with BMIs over 50 has not been completely addressed and it should be considered separately from patients with morbid...

20 January 2021 2,748 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View