Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,252 4 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,057 1 View

How can I interpret the negative value of regression coefficient in logistic regression??

I am running a logistic regression by using dichotomous dependent variable and five independent variable. I found one of the independent variable is getting -ve regression coefficient. 1) What...

07 November 2017 4,464 9 View

How Gray level co-occurrence matrix or GLCM calculated for an image is implemented on that image for texture feature extraction?

Information such as contrast, entropy, dissimilarity index and variance are computed to know the properties of any image, but how these values are used to for further classification or feature...

12 October 2017 4,978 3 View

Entering the field of Data Science as a developer

Being a python programmer, I have decided to get in the field of data science and later on AI and Machine Learning. I have already brushed up the knowledge of statistics and other python libraries...

23 August 2017 5,999 13 View

What are the assay I should use for insulin receptor activation on NIT cell lines?

We have NIT cell lines, what are the assay should I use for checking insulin receptor activation ?

10 May 2017 9,836 1 View

What assay I should perform for insulin receptor activation?

I want to check anti diabetic effect by insulin receptor activation. Which assay i could perform to check insulin receptor activation.

26 April 2017 1,835 2 View

Is atmospheric correction mandatory for chlorophyll estimation from Landsat images and which is best method of atmospheric correction?

I am trying to estimate chlorophyll-a by using Landsat/ MODIS image. So I want to know atmospheric correction for the above analysis is required or not? There is so much technique for atmospheric...

08 March 2016 9,488 2 View

How can I calculate the load of a virtual machine?

In a virtual machine there is a migration. Let's suppose i want to select a virtual machine, based on it's current load, and make a decision based on the current load to migrate virtual machine....

06 September 2013 9,969 18 View

How to measure OFDM PAPR in Matlab Simulink?

01 January 1970 7,579 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View