Do forests play any role in controling pandemics?

Only a decade or two ago it was widely thought that tropical forests and intact natural environments teeming with exotic wildlife threatened humans by harboring the viruses and pathogens that lead...

25 March 2020 1,727 6 View

Do forests play any role in controling pandemics?

Only a decade or two ago it was widely thought that tropical forests and intact natural environments teeming with exotic wildlife threatened humans by harboring the viruses and pathogens that lead...

25 March 2020 2,119 4 View

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

01 August 2019 8,465 4 View

How do I remove PCR inhibitors from a DNA elute?

I am currently extracting DNA from dried blood spots using the QIAamp DNA blood mini Kit. So far, the DNA concentration of my elute has been ~2-5 ng/uL with a 260/280 ratio of 1.8-2.5. Upon...

24 April 2018 9,122 9 View

Why is there no PCR gene amplification on the DNA isolated from dried blood spots?

I have isolated gDNA from human dried spots with a yield of 13.8ng/uL and a purity (260/280) of 1.82. However I observe that these isolated DNA is not amplifying in PCR. Can anyone tell me why...

15 April 2018 1,051 8 View

How to determine protein stability using GROMACS?

10 January 2017 6,224 17 View

What is importance of OXT atom found in aspartic acid? Does it participate in salt bridge formation?

During a molecular dynamics simulation i have found salt bridges being format being N(eta) atoms of Arg and OXT atom of Asp. What is the role of OXT atom and is such a salt bridge formation possible?

03 September 2016 3,929 3 View

Calcium as a chaotrope or kosmotrope?

17 May 2016 471 6 View

Can you turn Electrocompetent E.coli into Chemically Competent cells?

I want to buy a strain of cells but they only sell them as electrocompetent cells. I was wondering if I could make my own stock of calcium competent cells using the electrocompetent cells.

25 February 2015 1,453 2 View

How to study or determine horizontal gene transfer using bioinformatic tools?

Is it possible to study or determine horizontal gene transfer or the signs of HGT in evolution using sequence alignment or any other bioinformatics/in silico tools?

29 May 2014 8,026 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Which is the most ecofriendly and effective activated agent for the preparation of activated carbon from sewage sludge?

I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...

07 February 2021 9,507 5 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View