Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

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Cyclization should be performed before mutation or after mutation of a linear peptide sequence by in-silico method?

I have a doubt on in-silico modeling. I want to cyclize a linear peptide sequence by in silico. Which is more favorable method I don't understand. There is 2 pathways- 1. I can cyclize a linear...

12 June 2024 2,449 2 View

What cDNA dilution can be used for qPCR reaction?

I have obtained 100 ul of pre-amplified cDNA using the SSIV SINGLE CELL PRE-AMP KIT. Now i want to make a dilution because my samples are limited and i cannot directly use the cDNA. So what...

22 May 2024 6,017 3 View

How can I calculate average dG MM/GBSA energy from selected frames of MD trajectories?

I need to calculate dG MM/GBSA energy through the MD simulation trajectories. From RMSD values I selected last 400 frames from MD trajectories. I ran thermal_mmgbsa.py script, but it calculates dG...

22 May 2024 10,076 5 View

Is it necessary to change the medium every 24 hours after drug treatment in cell line study and provide fresh drug to the cell?

I am keeping my cell line for drug treatment and monitoring its effect at 24 hrs, 48 hrs and 72 hrs? Should I change the medium every 24 hrs and give fresh drug treatment or just leave the medium...

01 May 2024 10,011 3 View

Can anyone explain the N/P charge ratio of protein DNA complex during LLPS formation?

Protein:DNA charge ratio plays an important role in forming condensates. In most cases the N/P ratio ~1 is favored. However how this ratio depends on the concentration of the protein is not...

20 April 2024 4,753 0 View

How BLUE ECONOMY can upgrade the economic profile of the nations?

The "blue economy" refers to the sustainable use of ocean resources for economic growth, improved livelihoods, and jobs while preserving the health of ocean ecosystems. It encompasses a wide range...

19 April 2024 7,456 2 View

What could be the possible reason for this different microstructure?

I've prepared the following ceramics: Sample ID = Composition LMT = Li2Mg3TiO6 LMCT = Li2(Mg2.88Ca0.12)TiO6 LMCT-MN = Li2(Mg2.88Ca0.12)(Ti0.95(Mg1/3Nb2/3)0.05)O6 LMT-MN =...

07 April 2024 4,717 2 View

How AI can assist in the progress of Oceanography?

AI has immense potential to address various aspects of climate change by enhancing our understanding of the climate system, optimizing resource usage, and developing innovative solutions. Here are...

26 March 2024 958 1 View

How to homogenize gizzard tissue using Ultrasonic cell crusher?

I want to extract RNA from chicken gizzard tissue. But currently I don't have a homogenizer. What parameters should I use, like the power, Ton, Toff, total time etc, to effectively homogenize the...

29 February 2024 8,155 0 View

Mice Calprotectin ELISA kit (Stool)?

Have anyone tried to measure calprotectin from feces sample of mice using ELISA

03 February 2024 6,049 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Normality assumption for linear regression is The assumption of normality is whether for residual errors or predictor variavble?

When we conduct linear regression, there are several assumptions. The assumption of normality is whether the residual errors are normally distributed, not whether a predictor is normal?

31 July 2024 6,164 3 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View