The measures on how to apply the same frame work to my topic.
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...
08 July 2024 1,900 1 View
I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...
24 June 2024 6,079 4 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...
20 June 2024 892 3 View
Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...
17 June 2024 8,165 7 View
I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?
06 June 2024 5,182 2 View
Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...
21 May 2024 9,035 0 View
Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...
20 May 2024 4,277 0 View
I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...
19 May 2024 4,236 5 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?
28 July 2024 2,988 0 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
I have used 30 years of daily data, but the model is not able to optimize, getting just 0.57 in NSE and R2. when I reduced the data to 20 years in daily time frame I got 0.73 in NSE, R2 for both...
22 July 2024 669 0 View
"Dear Researchers, I am currently working on a Nanotechnology project and am exploring the process of drafting a patent to protect our innovations. Could anyone here share insights or tips on how...
02 July 2024 9,627 2 View
how to observe the hysteretic energy consumption for a parrticular frame element
12 June 2024 3,426 1 View
Hi, I am calculating the periods of a steel building modeled as a 3D moment frame. Is there any script to display the shape of vibrating of the model in opensees?
12 June 2024 8,065 1 View
Hi, I am running an opensees model of a 2-bay 1-storey frame with settlement at the mid-support. I wanted to add an additional frame element as a bracing to the analyzed frame after it sustains...
08 June 2024 4,132 0 View
formulate Phosphorus 52 and Potassium 34 in liquid
03 June 2024 7,589 2 View
I am dealing with some finicky antibodies that like to self adhere a bit in certain buffers. Working towards formulation for dosing we are trying to find good buffers that will minimize this...
30 May 2024 6,490 3 View