The measures on how to apply the same frame work to my topic.
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....
15 February 2021 5,833 3 View
Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...
08 February 2021 346 7 View
I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...
08 February 2021 2,673 1 View
Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...
07 February 2021 6,973 3 View
Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...
07 February 2021 3,643 4 View
Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...
31 January 2021 6,771 1 View
Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...
03 March 2021 9,989 2 View
For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...
24 February 2021 1,265 6 View
during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...
22 February 2021 380 5 View
Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...
22 February 2021 8,132 6 View
Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...
19 February 2021 4,562 4 View
Hi everyone, I'm working on an action prediction (i.e., early recognition) using Bag-of-Words. I know how to use BOW, but regarding action prediction, there is a subtle problem. In the...
19 February 2021 1,442 4 View
Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...
18 February 2021 9,581 2 View
Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...
15 February 2021 8,982 2 View
I am working on one optimization problem. the model with two states, the transition between the two states based on one threshold value (L = 5, which L< 5 -> Normal state, L== 5 ->...
11 February 2021 4,045 3 View
I have a trajectory file of about 1000 frames which I have calculated a center of mass for each frame. I would like to use the first frame as a reference and see how much the center of mass...
04 February 2021 5,774 1 View
