What are the software that can be used to analyse the best housekeeping genes across 3 different samples (Untreated+treated drug 1+treated drug 2)?

05 May 2016 1 9K Report

I am looking for the software that can analyse the qPCR data to find out the best housekeeping genes within 3 samples. I have untreated cell line, drug 1 treated cell line and drug 2 treated cell line. Any recommendation?

Seyit Ali Kayis

Hi in-Nee,

You can use geNorm, NormFinder or BestKeeper. The first one is widely used. Alternatively, depending on your experimental design (including technical replicates etc.) you can build up your model as well. 10.1016/j.anireprosci.2011.02.019 is an example for that.

Kind Regards

Seyit Ali

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