Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

How can I remove particles from a PDMS mold?

I have some particles that are about 300 micrometers tall in a PDMS mold. I'd like to collect these particles from the mold. I've tried using tape, and although it works, removing the particles...

17 July 2017 6,693 1 View

What are the software that can be used to analyse the best housekeeping genes across 3 different samples (Untreated+treated drug 1+treated drug 2)?

04 May 2016 8,843 1 View

How to solve low % Bootstrap on the phylogenetic tree?

16 February 2016 9,814 5 View

Which dialysis tubing molecular weight cut off (MWCO) should I use for bacterial exopolysaccharide?

01 February 2016 933 1 View

How to solve low % Bootstrap on the phylogenetic tree?

01 February 2016 2,102 6 View

On the phylogenetic tree, is it possible to have a scale bar of 5?

I just ran phylogenetic tree analysis of 16S rDNA sequences (around 1,500 bp) of Bacillus spp. using MEGA-6 and used Maximum Likelihood method with Bootstrap 1,000 replications and got the tree as...

11 December 2015 1,051 12 View

Why do I not get attache cells after while isolating neural stem cell from periventricular tissue?

I have been trying to isolate neural stem cells from periventricular tissue (taken from an adult patient). But I do not get any attached cells after 2-3 weeks of culture in NB medium supplemented...

31 December 2014 1,721 6 View

Interaction between light spins and electron spins ?

On your paper "Extended pump-probe Faraday rotation spectroscopy of the submicrosecond electron spin dynamics in n-type GaAs", I believe that the light spin (the continuous spin obtainable from...

01 January 1970 6,317 1 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

How to use multiple tables to generate a graph in Graphpad Prism?

I have two different tables: one for metabolic activity of the cells in a plate, the other one is the Total DNA assay from the same plate. I need to generate a graph which represents metabolic...

29 January 2021 3,294 5 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

How to calculate the number of degrees of freedom in a Mantel test?

Hello, I've performed a Mantel test between my microbiological data and the geographic distance where those samples were collected, using a table with 516 samples. However, the test doesn't...

16 January 2021 8,713 4 View

Which statistical test to use for comparing percentages between multiple groups?

Hello everyone! I am looking for some statistical help! I am working in in vitro micropropagation and one of the aspects that I am studying is the rooting of plants in 10 different growing media....

14 January 2021 7,575 5 View

How to determine Viscoelastic and Hyper Elastic Material Properties for Abaqus by mechanical testing ?

I am studying about Impact load resistance property of Polyurea (Elastomer). With respect to this, I need to conduct tests on this material to evaluate its material model behavior as a...

29 December 2020 2,969 1 View

Problems in Simulation of Carbon Dioxide discharge from pipe using fluent (Read description below)?

I am trying to simulate carbon dioxide discharging from the pipe using ansys fluent and want pressure variation with time inside the pipe. The geometry consists 144m long pipe and 146.36mm inner...

25 December 2020 1,533 3 View