I got a flake of white precipitation along with CTAB coated gold nanorod (790nm - 830nm) after centrifugation. I want to know the reasons behind it and can I still use it for in vivo, in vitro experiment?
Do you know the CTAB concentration of your solution ? If it is quite high, the precipitate you observe might be due to the crystallization of CTAB. CTAB can crystallise from aqueous solution, as it is not really dissolved in the solution, it is in the form of micelles. When it crystallises, it forms white "shiny" flakes. At what temperature you perform your centrifugation ? If it is at a relatively low temperature it may induce the cristallization of CTAB.
I used 0.2 M CTAB for seed solution and 0.05M for growth one. Regarding your answer, I warmed the solution up around at 400C and centrifuged again, but it didn't work at all. I am just worrying how can i fix it?
What do you mean by "it didn't work at all ?" What occured upon warming ? Did the flakes disappear ? Did they appear again upon cooling down ? Or did you observed again the formation of white flakes ? Did you attempt to collect the flakes+particles and add some water to disolve flakes and perform a second centrifugation ?
I didn't add more water but just warmed it under stirring (at 400C). After then, when I centrifuged for second time, I saw the same amount of flakes again as a pellet along with GNRs(the flakes are not shiny). As I have used 5-bromo Salicylic Acid as an additive in growth solution, I thought it may be this one but still getting confused as it didn't get dissolved.
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