Hi all,
I have been reading about quantification methods of sericin and fibroin. There are methods reported like FTIR, BCA, amino acid hydrolysis etc. I don't understand how it relates to exact sericin/fibroin titers in an isolate.
Does any body know of using or read about methods that are more accurate like ELISA or western for quantification of sericin or fibroin in protein isolate?
1. Ultraviolet Spectrophotometry: This is a method used to quantify sericin and fibroin by measuring the absorption of ultraviolet light by the proteins. 2. High Performance Liquid Chromatography (HPLC): This method involves passing a sample of sericin and fibroin through a column that is containing a stationary phase and a mobile phase. The concentration of the proteins can then be determined by measuring the changes in the absorbance of the mobile phase. 3. Mass Spectrometry: Mass spectrometry is a technique used to measure the mass of a molecule, which can then be used to calculate the amount of sericin and fibroin present in a sample. 4. Immunoassay: This method uses antibodies that are specific to sericin and fibroin to quantify the proteins. The amount of antibody-protein complex formed can then be measured and used to determine the concentration of the proteins.
While I appreciate your efforts to assist me, I would like to kindly request that you refrain from adding answers that come from AI-based model.
As you may know, I am here to gain a practical scientific approach to deal with these problems from people who are skilled and experienced in that area.
Bandla Ramesh Therefore, I am seeking the knowledge and insights that come from real-life experiences and expertise, rather than solely relying on machine-generated responses.
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