Bhawna G.

9 Questions 6 Answers 0 Followers

Questions related from Bhawna G.

Trouble cloning of a bacmid miniF replicon. The fragment seems to be ligated but only some part of the fragment is recovered from the plasmid?

Hi, I have been trying to clone 8.4kb miniF replicon from bacmid. I have tried doing that in pFastBAc backbone but the fragment seems to be lost after every trial. While doing colony PCR, I am...

19 February 2024 1,172 1 View

Does anyone have vector map of pBKBlue plasmid?

I have been reading this paper, Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells...

16 December 2023 8,548 1 View

Suggestion on strategies to make multiple gene deletions to reduce the vector size?

Hi, I want to delete multiple regions from a viral vector to reduce the size of the same. What I have in mind is homologous recombination but that would need me to swap it with another DNA...

27 September 2023 1,491 3 View

Has anyone assembled 120 kb or more synthetic constructs using GIBSON or In-fusion cloning kits?

Hi, 1. Does any one know what is the maximum length of amplicons we can amplify using PCR? I need to amplify fragments for assembling a big vector (approximately 100kb). 2. Do you think it's...

08 September 2023 2,356 1 View

Why HeraLabs supply piggybac transposase as a non autonomously replicating plasmid?

Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...

18 April 2023 5,842 6 View

What are the quantification methods of sericin and fibroin ?

Hi all, I have been reading about quantification methods of sericin and fibroin. There are methods reported like FTIR, BCA, amino acid hydrolysis etc. I don't understand how it relates to exact...

31 January 2023 3,834 2 View

Alternatives or more accurate methods of proximate analysis of isolates and concentrates?

Hi, I'm currently working on insect-based products. All the methods of quantifying carbohydrates, proteins, fibers, fats etc that I have come across with so far are by proximate means. Has anyone...

19 January 2023 3,565 3 View

How to set pH of Invitrogen Fermentation Media of Pichia Pastoris?

Hii, I'm working with Pichia pastoris SMD1168 Strain. To proceed with fermentation I want to maintain pH around 5.5~6, however the fermentation media suggested by Invitrogen gets precipitated over...

28 July 2021 1,435 4 View

Is it oxygen limitation or nutrient deficit causing the growth curves to be non linear after OD 1?

Hello all, I've observed that OD vs time curve tends to be non linear after OD 1. Since the observation is consistent for 3 strains (E.coli, Saccharomyces cerevisiae and Pichia pastoris) growing...

04 April 2021 1,012 2 View