17 October 2024 2 6K Report

I am trying to detect cereulide toxin produced by Bacillus cereus bacteria strains using HPLC method, in which i have chosen Valinomycin as a standard sample of reference. but then i don't know where tostart

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A good or great tittle for Hydraulic press?

I wand to do research project of Hydraulic Press, I need your help regarding and how can I conduct this research as I don't have much knowledge of research project.

14 March 2023 4,808 8 View

Steam/liquid water calculations in steam methane reforming reactions?

For a molar ratio of CH4:H2O being 1:0.5 in steam methane reforming reactions, if 25ml/min CH4 is fed, what is the corresponding flow rate of liquid water (not steam) needed?

02 February 2023 9,079 1 View

How are constraints applied to study membrane diffusion using adaptive biasing force?

Hello, I am planning on measuring the energy associated with diffusion of small molecules through a membrane using ABF. I have generated the initial reaction coordinate using steered MD (along the...

22 January 2023 6,011 0 View

Why could there be an unwanted 30 amino acid insertion in my mutagenesis product?

I have been performing site-directed mutagenesis with the goal of substituting different amino acids. I have done this successfully three times but when I was attempting my fourth sequencing...

10 July 2022 7,990 3 View

Is there an enzyme that will catalyze the ligation of an aminoacylated RNA to the 5' end of another RNA?

Hello all, I was wondering if I had a pool of aminoacylated RNA oligos with an amino acid covalently attached to their 3' end, and a pool of "target" RNAs, is there an enzyme that could ligate the...

26 June 2022 9,124 1 View

Parameters for ultrasonic removal of surface bacteria?

Hello, I wish to isolate phyllosphere bacteria from sample leaf surfaces using a commercial ultrasonic bath. Most sonicators in relevant literature operate at 40kHz for 8-10 minutes, but very...

10 March 2022 6,274 4 View

What could this CD45-, CD11b+ population be?

I'm looking at Helios CyTOF data of virally-infected mouse brains at day 7 post-infection. I've got this large population of CD45-, CD11b+ cells. I'm pretty confident that they're negative for...

04 January 2022 4,737 0 View

How do I interpret the y-axis of a Faith's phylogenetic diversity plot?

Hi all! I'm brand new to microbiome data analysis, so any help here is appreciated. My understanding is that the y-axis is a measure of the sum of the branch lengths for all members in whatever...

18 April 2021 1,386 3 View

How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,276 3 View

Is there a quick insulin activity test?

My 3T3L1 adipocytes are not responding to insulin properly in recent experiments. I am wondering if my insulin stopped working. Is there a quick test for this? I normally use 100 nM for 20 min and...

01 December 2020 5,383 9 View

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A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

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Can I use a HisTRAP column for affinity chromatography?

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Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

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How can we use mobile apps for improving students' academic performance?

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