I am combining drug A (IC20 and IC50) with drug B (5 uM, 10 uM and 50 uM). Drug B reduces cell viability to 28% at 50 uM, while drug A IC20 combined with drug B 50 uM reduces cell viability to 32%. CompuSyn gives a CI=0.46. Is this correct? To have synergism shouldn't the viability be reduced to less than 28% when combining the 2 drugs at these concentrations?
Thanks for any help.
Atul R Bendale
You can use the combination index, which is a quantitative measure of drug interaction. It is calculated using the formula
CI = (D1/EDx1) + (D2/EDx2),
where D1 and D2 are the doses of drugs 1 and 2, and
EDx1 and EDx2 are the doses that produce x% effect individually.
A CI value less than 1 indicates synergism.
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