What are the possible methods for identifying and measuring Glycans in the blood?

More Milad Moloudizargari's questions See All

Has anyone worked on exosome release?

What are the best methods to study the alterations in exosome release from a specific cell line following treatment with an agent? What if the intended agent changes the viability of the treated...

02 March 2018 1,452 8 View

Protocols for NK cytotoxicity and degranulation?

I need two detailed and reliable lab protocols one for assessing NK cytotoxicity and one for NK degranulation assay using flow cytometry. Is NK cytotoxicity done only in co-culture with K562 or...

01 February 2018 2,141 12 View

Experienced researchers familiar with flow cytometry?

Please take a look at the attached files: I am about to study 2 markers which appear in FL1 and FL2; In the unstain tube, I see 2 distinct populations one of which has weaker auto-fluorescence...

10 November 2017 6,585 7 View

What are novel adjuvants for tumor immunotherapy with the potential to be used in clinical trials?

I`m about to start my thesis on breast cancer immunotherapy which is designated to examine a nano particle-based vaccine for the delivery of tumor associated antigens to DCs in vivo. However, I...

08 September 2014 7,232 2 View

Why do the suitable voltages for my unstain and control differ completely from my treatment groups?

I`m performing a flow cytometric analysis to study apoptosis in peripheral blood mononuclear cells (PBMCs) of patients using Annexin V-FITC (FL1) and Propidium Iodide (FL2). My tubes include and...

01 January 1970 6,167 20 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

15 July 2024 762 3 View

Oncologist at Visakhapatnam interested to collaborate?

Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...

14 July 2024 936 0 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View

WST-1 test for 3D cell culture?

I want to perform WST-1 test with alginate based 3D-colorectal cancer cells. I wonder about before performing WST-1 test, ıs it necessary to remove alginate spheres from the cells (to carry it...

29 June 2024 9,667 1 View

Elbay Malikmammadov

The easiest way is lab-on-a-chip method.

Milad Moloudizargari

could you please elaborate more on it Mr. Malikammadov?

well, it actually depends on whether you have to do a medical experiment or research-based experiment. For medical ones, lab on-a-achip is the best method, since it is fast and reliabe. But if your study is research-based, then you can use just centrifuge + chromatography or centrifuge + uv-vis spectroscopy (with a flourescent agent for ex)

Thank you Sir, so helpful