06 April 2014 1 5K Report

For antibiotic tablets and amino acid capsules formulations.

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Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

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Can review and Cite?

Dear all, Can you go through the research article links and review and cite. https://scholar.google.com/citations?user=bKF5yGsAAAAJ&hl=en&authuser=2 Happy if connecting with researchers to...

05 August 2020 8,278 2 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

Does anyone have the Grass Instruments/AstroMed S88X Stimulator Manual?

Hello Everyone, Does anyone by chance have a Grass Instruments/Astro Med S88X stimulator manual? (http://www.neurolabparts.com/stimulators/99-grass-astro-med-stimulators-model-s88x.html). I...

17 December 2019 1,447 1 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

13 October 2019 1,208 2 View

Does tumor studies in animal require 3 independent experiments done in 3 separate times?

I was having a discussion with my colleague regarding the idea of independent experiments. Is it that every mice with a distinct tumor an independent experiment or does independent experiment mean...

29 October 2018 6,949 2 View

How to run a simulation on an already simulated model or is it possible?

Lets say there is a model i created, and i am done running the simulation and have the results. Now, i need to do take it further by doing another simulation but on the current model, i.e. the...

24 June 2018 8,429 4 View

Looking for opensource tool for ReaxFF simulation. If there something other than paid software ADF please reply ?

Looking for opensource tool for ReaxFF simulation. If there is something other than paid software ADF please reply ?

24 January 2018 6,596 5 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

08 November 2017 5,895 11 View

How to clone a 5 kb insert to a vector successfully?

Hello, everyone. I'm trying to subclone a 5 kb gene to a vector (approx. 5 kb). Initially, I designed the primers which have restriction enzymes overhang (XhoI and XbaI). I amplified my gene using...

27 August 2017 4,722 4 View

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