I have a protocol from a research paper for performing this assay. I tried finding the original paper that provides a detailed protocol but I cant find one.
The protocol in the papers mention a step: Wash the erythrocytes with Ringer's solution atleast three times.
Here washing certainly means centrifuging and resuspending. So I want to know the speed (preferably in g), time and temperature at which the erythrocytes can be centrifuged without getting crushed and hinder with my assay.
Thank you very much for your suggestions.
Dear Omkar,
You can use protocol that described in our publication:
https://www.researchgate.net/publication/8247482_Flow-resistant_red_blood_cell_aggregation_in_morbid_obesity
Article Flow-resistant red blood cell aggregation in morbid obesity
Dear Omkar Panchal ,
Can you share title of the paper ? I can try to find .
Best regards,
Babu
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