Omkar Panchal

3 Questions 13 Answers 0 Followers

Questions related from Omkar Panchal

Can Calcein AM dye really differentiate between live and dead cells?

I am working with spheroid models. Cells of Head and Neck squamous carcinoma are seeded in special U bottom plates where they form spheroids. I treat my spheroids with Cetuximab and/or...

27 September 2017 5,500 2 View

Which genes other than Cyclin B1 and D1 should be analysed to check the effect of your treatment on the rate of cell cycle?

In one of the cell line, the knockdown of my target gene (SATB1) shows an upregulated transcription of Cyclin B1 and D1 (q-RT-PCR). However, cell cycle analysis (flow cytometry) by Nocodazol block...

26 April 2017 990 4 View

What are the parameters for washing the erythrocytes with Ringer's solution for performing erythrocyte aggregation assay?

I have a protocol from a research paper for performing this assay. I tried finding the original paper that provides a detailed protocol but I cant find one. The protocol in the papers mention a...

26 January 2015 3,980 2 View