monitoring gene expression has been resolved but, assigning functions is still an issue
model plants for genomics study in dicots is another issue
Still now the customer didn't eat the GMO production in plant or animals or cotton wear also.
So, big equation now. How we can derived the GMO to get customer's trust ???!!!
monitoring gene expression has been resolved but, assigning functions is still an issue
model plants for genomics study in dicots is another issue
One issue is the function of the non-coding DNA inside and outside the genes that both constitute the vast majority of human DNA (about 98%).
One other issue is the complex interaction of genes especially disease-related ones. Many of them are still unknown.
Although the informations about human genomics are comming to a great extent after the genome sequence revealed but variations among indevidual genes have remain elusive. More gemomics research are needed to completely execute the concept of personalized medicine.
I must sadly and repeatedly say that notorious Genetics is not surely working in the humans at all. Humans are only fighting against virus and bacteria.
Recently I have found that Down syndrome is caused by infection of Human adenovirus/HAdV (linear dsDNA virus, without envelope) and/or Human poliovirus/PV (+ssRNA, without envelope) during pregnancy (my unpublished result). Only three specimens have the Down syndrome cell adhesion molecule/CHD2/DSCAM (a membrane glycoprotein). Inducing power for Down syndrome cell adhesion molecule by HAdV-6 seems to be weak as compared to HAdV-2 and HAdV-40.
Hepatoma HepG2 has Down syndrome cell adhesion molecule/CHD2/DSCAM at 6.8 μg/mg cell protein. This cell has Packaging protein 3 (HAdV-2) at 0.2 μg/mg cell protein and Genome polyprotein (PV) at 5.1 μg/mg cell protein.
Healed hepatocyte HepG2 (cultured with fucoidan at 0.102 mg/mL for 3 days) has Down syndrome cell adhesion molecule/CHD2/DSCAM at 5.6 μg/mg cell protein. This cell has DNA polymerase (HAdV-40) at 1.3 μg/mg cell protein.
Serum of 1y girl (with biotin deficiency) has Down syndrome cell adhesion molecule/CHD2/DSCAM at 9.8 μg/mg serum protein. This serum has Maturation protein (HAdV-6) at 7.7 μg/mg serum protein.
Down syndrome cell adhesion molecule/CHD2/DSCAM shows up legulation of phosphorylation of proteins.
Thus, I would like to recommend the new quantitative proteomics PDMD method (please see files; HepG2 fucoidan and JMBT Alopecia).
Furthermore, HAdV seems to up regulate biotinidase (please see file; Brain-BIN Pig) and Brain-development proteins in addition to Down syndrome cell adhesion molecule/CHD2/DSCAM; as foolows; i.e.,
Liver tissue (with Pseudo-liver cancer) has HAdV at 2.0 μg/mg tissue protein. This tissue has Homeobox protein Hox-B7/Homeobox protein Hox-2C at 1.3, Semaphorin 3F at 0.76, Kidney biotinidase at 16.0, and Serum biotinidase at 22.7 μg/mg tissue protein, respectively. Total biotinidase and Brain-development protein becomes to be 40.76 μg/mg tissue protein.
LC tissue (with leprosy) has HAdV at 2.9 μg/mg tissue protein. This tissue has Cerebellin-1/Precerebellin at 1.4, Homeobox/POU domain protein RDC-1/Brain-specific homeobox/POU domain protein 3A at 1.6, Kidney biotinidase at 13.0, and Serum biotinidase at 23.0 μg/mg tissue protein, respectively. Total biotinidase and Brain-development protein becomes to be 39.0 μg/mg tissue protein.
HCC tissue (with PBC) has HAdV at 0.08 μg/mg tissue protein. This tissue has Synapsin-1/Synapsin I/Brain protein 4.1 at 3.4, Kidney biotinidase at 8.3, and Serum biotinidase at 11.5 μg/mg tissue protein, respectively. Total biotinidase and Brain-development protein becomes to be 23.2 μg/mg tissue protein.
LC tissue (named as No.6) has HAdV at 2.6 μg/mg tissue protein. This tissue has Homeobox protein HOX-B7/Homeobox protein Hox-2C; at 0.6, Synaptic functional regulator FMR1/Fragile X mental retardation 1 protein/Protein FMR-1 at 1.5, Serum biotinidase at 25.6, and Kidney biotinidase at 15.2 μg/mg tissue protein, respectively. Total biotinidase and Brain-development protein becomes to be 40.8 μg/mg tissue protein.
HCC tissue (No.6) has HAdV at 4.6 μg/mg tissue protein. This tissue has Possible global transcription activator SNF2L/ATP-dependent helicase SMARCA1 at 5.2, Synaptotagmin 1/P65 at 1.6, Tyrosine-protein kinase Lyn at 1.3, Serum biotinidase at 25.4, and Kidney biotinidase at 9.5 μg/mg tissue protein, respectively. Total biotinidase and Brain-development protein becomes to be 43.0 μg/mg tissue protein.
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