Dear all,
What are the best methodologies to find out the ectomycorrhizal colonization on roots? I am looking for a publishable methodology to have a tuber sp. percentages of my seedlings.
Samiullah Khan
Root staining: This involves staining roots with dyes such as trypan blue, which will stain the fungal structures blue, allowing for visualization and quantification of ectomycorrhizal colonization. Root observation: This involves careful observation of the roots using a microscope to identify and quantify ectomycorrhizal colonization. Molecular techniques: PCR-based methods such as quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) can be used to quantify fungal DNA in roots, providing a measure of ectomycorrhizal colonization. Image analysis: Images of root systems can be captured and analyzed using software to quantify the percentage of roots colonized by ectomycorrhizal fungi.
There are several methods to quantify ectomycorrhizal colonization on roots, including:
It's important to note that different methods may yield different results and that the choice of method will depend on factors such as the specific research question, the resources available, and the expertise of the researcher. Additionally, it's important to follow established protocols and to use appropriate controls to ensure the accuracy and reliability of the results.
1 votes
0 thanks
- Badges
- Science method
More Mahesh Galappaththi's questions See All
Similar questions and discussions