What are the methods to follow if I want to study cross talk between molecular pathways?

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All the experimental results need to be presented with statistical analysis. In case of adsorption isotherm and kinetic study, what does I include?

What are the things should I include under statistical analysis.

19 November 2020 5,098 2 View

How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

10 June 2019 6,950 2 View

Need a protocol for SOD, Catalase and LPO enzyme assay in 96 well plate?

I need detailed protocol. Thanks in advance.

20 June 2018 6,887 3 View

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19 June 2018 6,121 3 View

How to plot in-plane vibration modes of square plate using MATLAB. The plot looks like a distrorted X-Y plane grid ?

I have the natural frequencies and the related displacement equations in x, y directions for in-plane vibration. Every point in the plate has a displacement in x (denoted by u(x,y) ) and y...

30 October 2017 304 11 View

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

22 October 2017 3,767 6 View

Given a bare-Lagrangian for a renormalizable, interacting system, is the renormaized version unique? If yes, then which ?

It is well-known that several renormalized versions of a given theory may emerge depending upon the chosen regularization method, choice of subtraction scale/scheme,...

09 March 2017 8,192 40 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

Between ER and SQ grade reagents of Qualigens (Thermo fisher) which one is analytical grade [AR] reagent ?

23 November 2016 716 7 View

Why the ligand structure changes during molecular dynamics simulation?

I did MD simulation for 10 ns. the ligand was inside the simulation box upto 10ns. only in the 3rd ns the structure was ok. in all other time one of its double bond was missing. many times the OH...

23 December 2015 9,118 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View