James Andrew Thompson

4 Questions 5 Answers 0 Followers

Questions related from James Andrew Thompson

Typically, what antibody concentration would be used for immobilization in SPR experiments?

I am looking to do some SPR work using the capture method, which involves immobilization of an antibody onto a chip surface before capturing ligand. I have limited antibodies available and was...

05 January 2015 2,839 3 View

How do I find out where this peptide will bind to alpha V beta 6 integrin?

We have a peptide, made by the chemistry department, that they say should bind to the alpha V beta 6 integrin. How would I go about seeing where exactly the peptide will bind to the integrin? This...

06 November 2014 7,444 4 View

What would be the best method for immobilising an integrin?

I am planning on immobilising alpha-V beta-6 and am debating whether it would be best to buy (not sure where from) His- or GST-tagged dimer or to immobilise a lipid membrane that expresses the...

10 October 2014 5,800 3 View

What is the significance of including DDT in the running buffer of a surface plasmon resonance experiment?

I am currently carrying out work analyzing the kinetics of progesterone receptor/ligand interactions using SPR. Currently when capturing the progesterone receptor (PR), the running buffer does not...

13 March 2014 7,942 3 View