What are the Isolation and identification methods of arbuscular mycorrhiza fungi ?

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How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Does the China's adaption to GM soybean Technology contradicts to its practice of virtual water trade policy?

Recently, the Ministry of Agricultural and Rural Affairs enacted the approval of twelve genetically modified (GM) soybean varieties, marking a strategic pivot in China's quest for food security....

05 June 2024 1,143 0 View

How can I correct the orientation of GOI from one plasmid to another?

A plasmid of mine has GOI in opposite orientation. I have another plasmid which has exact same RE site but in such a way that it orients my GOI in right direction. However even after multiple...

05 May 2024 5,662 2 View

How to find covariance matrix in case of parameter estimation by using Recursive least square with forgetting factor?

Thank you for reading my question. Currently, I am doing parameter estimation of Lithium-ion battery by using RLSF Algorithm. I am getting all the parameters as negative value. Which is not...

17 April 2024 8,941 0 View

Can anyone advice me on LncRNA primer designing for cloning to study its overexpressed pattern in the cell?

I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as...

14 April 2024 3,966 3 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

Concentration of chlorate when analyzing decomposition products of sodium hypochlorite(Chlorine bleach)?

hello. I majored in biochemistry and do chemical registration consulting work. Recently, I have been working on chlorine bleach, and a problem has arisen in identifying decomposition products....

27 February 2024 7,496 3 View

Solvent for mucilage other than water?

Please recommend any organic solvent for dissolving mucilage.

30 January 2024 5,692 5 View

I have a mixed microbial suspension. I need to analyze all the enzymes, if any, present in the sample. Can anyone suggest which technique will work?

Which process or technique will work? Will LC-MS give me a full enzyme profile?

26 January 2024 3,374 1 View

Is there a significant impact on drug treatment outcome when conducted in a 6-well plate with pooled samples versus using a T25 flask?

Does it make a difference whether I conduct drug treatment in a 6-well plate, utilizing multiple wells and subsequently pooling the samples to achieve one million cells, or if I should opt for...

21 January 2024 9,405 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View