I was wondering if someone has optimised a way of producing some HIV with HIV patient PBMC as starting material.

What would be the best medium?

The best IL-2 concentration?

The best cell concentration (per mL and surface)?

The best way to activate them with CD3/CD28 coated beads (bead:cell ratio, time)?

How regularly should I change the medium and how much?

How often should I add fresh activated PBMC?

etc.

Similar questions and discussions