How can I decide on the best MOI for HIV production?

05 May 2014 1 7K Report

I was wondering how to choose the best MOI for virus production? Would it be 50% of wells infected at D5? Or 100% or else?

Bernd Zorr

For a single hit kinetic you will need approximately an MOI of 5-10/cell.Example: If you want to infect 10E6 cells, you will need approximately 5-10E6 PFU /ml. If you multiply the TCID50 titer with 0.7 to get the approximate number of PFU / ml.

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