Type of inactivation depends on what you want to do with the virus after inactivation, if you want to do an infection than heating will disrupt the structure of evelope glycoproteins and this virus will not be able to infect.

I want to inactivate it i.e to have "dead" virus, no more infectious particles.

Here is a nice paper about HIV inactivation and stability. The rationality is the same as with FBS decomplementation. Heating at 56 for 30min degrades the enzymatic activity. In the case of HIV-1, the enzymes (reverse transcriptase and integrase mostly) are required for infectivity but heat can also affect envelope protein integrity. We use PFA 2% for 20 minutes, Ethanol 70% for 20 minutes and we have also used 2-aldrithiol to inactivate the virus without affecting the conformation of envelope proteins. They all work very well.

http://onlinelibrary.wiley.com/doi/10.1002/jmv.1890350402/pdf

Thanks!

Thank you very much

Thanks !!!