I want to carry out a hydroponic culture with a simplistic device that can be carried out in a laboratory of wheat seeds to which I wish to subject salt stress and bacterial inoculation in order to stimulate growth under applied stress
Dear Yasmine: growing plants in hydroponic to study salt stress is not beneficial for it is different as in soils. The mobility of salts, adsorption, availability of water,etc... all work together to give different results. Regards.
First of all, thank you for the interest and the answer. I do not wish to study the effect of salt stress I want to induce salinity tolerance in wheat by halophilic bacteria
Dear Dr Yasmine,
greetings, Seed surface could sterilized with 0.2% Clorox for 10 minute and then rinsed five times in sterile distilled water. Seeds could sowed on a witted sterilized filter papers in sterilized Petri-dishes and incubated at 4ºC until germinate. Seedlings sub-cultured in plastic boxes containing Hoagland solution and maintained at the growth chamber under 16/8 hour light/dark condition (with white florescence) at 20ºC. After one week, ten seedlings from each variety were transferred to new plastic boxes containing Hoagland solution
Kind regrads
Khaled
Thank you for your interest and response.
can you tell me a bit more about Hoagland's solution.
THANKS
This may help...
https://biotecharticles.com/Agriculture-Article/Hydroponics-Cultivation-of-Wheat-4374.html
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