If I am studying the effect of a stress factor on a plant that is known to accumulate terpenoid Glycosides, what enzymes should I be looking for?

I will use gel staining to investigate the response of some plant to water stress. such plants known to accumulate terpenoid Glycosides, what enzymes should I stain?

02 March 2019 9,991 4 View

What motivate antioxidant enzymes in tissue culture media?

I know that cells under tissue culture conditions are different from those in vivo in many ways, but what exactly could trigger antioxidant enzymes such as POX, SOD and CAT in tissue culture media?

11 December 2017 8,008 4 View

How to add similarity scale (%) to dendrograms in spss?

19 February 2017 4,403 2 View

How to add similarity scale (%) to dendrograms in spss?

01 February 2017 4,522 2 View

Does anyone have a protocol for spectrophotometric determination of Lipase activity?

I want a protocol for spectrophotometric determination of Lipase activity in plant tissses, If anyone has such; please provide

05 June 2016 3,604 2 View

How to calculate percantage of total proteins in Lowry's method?

05 June 2016 3,084 5 View

Can anyone help, how to calculate the membrane stability index (MSI)?

Is there an equation to calculate it, does it have a unit?

04 May 2016 6,717 2 View

What is the fastest method to stain SDS-PAGEs other than the usual-time consuming CBB method?

Comassie Brilliaant Blue staining takes so much time If anyone have tried other methods with reliable results many thanks

04 May 2016 7,143 8 View

What are the formulas applied to calculate H2O2, MDA, POX, Total free amino acid and water soluble & insoluble proteins in plant tissues ?

01 February 2016 1,568 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View