I will use gel staining to investigate the response of some plant to water stress. such plants known to accumulate terpenoid Glycosides, what enzymes should I stain?
Have you checked out this page? Enzyme glucosyltransferases can be one of the key enzyme to look for.
Article Potential applications of glucosyltransferases in terpene gl...
Also see this paper: Article Enzymatic glycosylation of terpenoids
Abstract
A significant number of terpenoid compounds are glycosides with the sugars linked to the active groups. Sometimes, the glycosidic residue is crucial for their activity, but in other cases glycosylation only improves pharmacokinetic parameters. Enzymatic glycosylation of terpenoids is a useful tool due to the high selectivity and the mildness of the reaction conditions, in comparison with chemical methods. Several types of biocatalysts have been used in the enzymatic glycosylation of terpenoids. These include the use of glycosyltransferases, trans-glycosidases, and whole-cell biotransformation systems capable of regenerating the cofactor, such as fungi, bacteria, plant-cell cultures, etc. Many biosynthesized terpenoid glycosides display medicinal and pharmacological properties and can be used as pro-drug substances. These terpenoid glycosides have also been employed as food additives (e.g. low-caloric sweetener compounds) and cosmetics, and even have applications as controlled-release fragrances.
Measuring glycosyltransferases:
1. Paper #1 (From Biochemistry): Article Flexible design clinical trial methodology in regulatory applications
2. Paper #2 (from Methods Mol Biol): Chapter Methods for Determining Glycosyltransferase Kinetics
- Badges
- Science topic
- Similar topics
- Biological Science
- Physiology
- Physiological Processes
- Stress