06 June 2016 2 4K Report

I want a protocol for spectrophotometric determination of Lipase activity in plant tissses, If anyone has such; please provide

More Abdullah El-nagish's questions See All
If I am studying the effect of a stress factor on a plant that is known to accumulate terpenoid Glycosides, what enzymes should I be looking for?

I will use gel staining to investigate the response of some plant to water stress. such plants known to accumulate terpenoid Glycosides, what enzymes should I stain?

02 March 2019 10,072 4 View

What motivate antioxidant enzymes in tissue culture media?

I know that cells under tissue culture conditions are different from those in vivo in many ways, but what exactly could trigger antioxidant enzymes such as POX, SOD and CAT in tissue culture media?

11 December 2017 8,103 4 View

Is it true that cotyledonary node are not recommended to use for in vitro multiplication when true-to-type is the purpose of the work?

Greetings to all researchers! Can anyone confirm weather it is true or not that cotyledonary node are not recommended to use for in vitro multiplication when true-to-type is the purpose of the...

12 March 2017 7,791 5 View

How to add similarity scale (%) to dendrograms in spss?

I have the one-zero file from analyzing the gel, I use spss to generate dendrograms from the Analyze-Classify-hierarchical analysis. How to add similarity scale as shown in the picture below  to...

19 February 2017 4,487 2 View

How to add similarity scale (%) to dendrograms in spss?

I have the one-zero file from analyzing the gel, I use spss to generate dendrograms from the Analyze-Classify-hierarchical analysis. How to add similarity scale as shown in the picture below  to...

01 February 2017 4,628 2 View

What are the genetic and epigenetic changes that lead to somaclonal variations in plants?

apart from DNA methylation, what also could be considered as an epigenetic changes that cause somaclonal variations in prolonged micropropagted plants? Are there any other genetic changes that...

09 October 2016 1,858 2 View

How to calculate percantage of total proteins in Lowry's method?

Hi everyone: I followed the steps known wt of tissue is homogenized with a certain volume of 0.1 N NaOH for at least 1\2 hour. Then 1 ml of this homogenate was added to 5 ml of alkaline reagent...

05 June 2016 3,179 5 View

Can anyone help, how to calculate the membrane stability index (MSI)?

Is there an equation to calculate it, does it have a unit?

04 May 2016 6,896 2 View

What is the fastest method to stain SDS-PAGEs other than the usual-time consuming CBB method?

Comassie Brilliaant Blue staining takes so much time If anyone have tried other methods with reliable results many thanks

04 May 2016 7,250 8 View

What is the equation applied to calculate the activity of SOD "super Oxide Dismutase" in plant tissues?

I have the Abs. values and i want to convert it to mg/g FW or any other unit, the reaction mix was riboflavin, L-methionine and NBT.  

29 April 2016 8,314 5 View

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