Apart from the names can anyone give me insights about the function of these (preferably in mammalian systems)? Particularly I'm interested in any relations to translational initiation (as I found my only two start-codons flanked by these).
I think you are asking the wrong person with the same name. There's another M Shariati in your field.
Good luck
Does anyone have good experiences with viral delivery to the oral cavities of mice i.e. high efficiency transduction of the tissue?Thanks, Kristian
06 July 2016 143 1 View
If infectious lentiviral particles are injected intravenously into a mouse, how will they distribute into various tissues, and is that mode of delivery even efficient?
01 February 2016 4,769 2 View
Transcript levels are frequently taken as a measure of gene activity, but other factors such as transcript stability also affect the levels of transcript. What data would be sufficient to...
10 November 2013 7,854 8 View
Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...
07 August 2013 8,000 4 View
Is it valid to normalize gene expression to the average between biological repeats? Could that approach be used for large data sets were most genes show some degree of variation or is there a...
10 November 2012 7,773 7 View
Anybody has a good recipe for homemade SYBR green mix for real time PCR? To my understanding, one of the key factors is the taq enzyme because most enzymes are inhibited by the SYBR dye.
10 November 2012 7,102 1 View
Does anyone have a good recipe for homemade Sybr Green Master mix? I have tried a couple, and to my understanding the brand/variant of the DNA polymerase is a major factor. Any experiences as to...
10 November 2012 4,440 9 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View