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Questions related from Kristian Bruun Laursen
Does anyone have good experiences with viral delivery to the oral cavities of mice i.e. high efficiency transduction of the tissue?Thanks, Kristian
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If infectious lentiviral particles are injected intravenously into a mouse, how will they distribute into various tissues, and is that mode of delivery even efficient?
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Apart from the names can anyone give me insights about the function of these (preferably in mammalian systems)? Particularly I'm interested in any relations to translational initiation (as I found...
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Transcript levels are frequently taken as a measure of gene activity, but other factors such as transcript stability also affect the levels of transcript. What data would be sufficient to...
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Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...
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Anybody has a good recipe for homemade SYBR green mix for real time PCR? To my understanding, one of the key factors is the taq enzyme because most enzymes are inhibited by the SYBR dye.
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Is it valid to normalize gene expression to the average between biological repeats? Could that approach be used for large data sets were most genes show some degree of variation or is there a...
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Does anyone have a good recipe for homemade Sybr Green Master mix? I have tried a couple, and to my understanding the brand/variant of the DNA polymerase is a major factor. Any experiences as to...
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