Does anyone have good experiences with viral delivery to the oral cavities of mice i.e. high efficiency transduction of the tissue?
Thanks, Kristian
we delivery virus into the lung via the mouth of the mice.
If infectious lentiviral particles are injected intravenously into a mouse, how will they distribute into various tissues, and is that mode of delivery even efficient?
01 February 2016 4,895 2 View
Apart from the names can anyone give me insights about the function of these (preferably in mammalian systems)? Particularly I'm interested in any relations to translational initiation (as I found...
06 July 2014 5,418 1 View
Transcript levels are frequently taken as a measure of gene activity, but other factors such as transcript stability also affect the levels of transcript. What data would be sufficient to...
10 November 2013 7,962 8 View
Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...
07 August 2013 8,121 4 View
Does anyone have a good recipe for homemade Sybr Green Master mix? I have tried a couple, and to my understanding the brand/variant of the DNA polymerase is a major factor. Any experiences as to...
10 November 2012 4,572 9 View
Anybody has a good recipe for homemade SYBR green mix for real time PCR? To my understanding, one of the key factors is the taq enzyme because most enzymes are inhibited by the SYBR dye.
10 November 2012 7,235 1 View
Is it valid to normalize gene expression to the average between biological repeats? Could that approach be used for large data sets were most genes show some degree of variation or is there a...
10 November 2012 7,923 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
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May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
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My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
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Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
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A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
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