Intravenous lentivirus delivery?

If infectious lentiviral particles are injected intravenously into a mouse, how will they distribute into various tissues, and is that mode of delivery even efficient?

01 February 2016 4,769 2 View

What are the functions of DNA polymerase arrest sites (WGGAG) and Hamster deletion hotspot sequence (TGGAG)?

Apart from the names can anyone give me insights about the function of these (preferably in mammalian systems)? Particularly I'm interested in any relations to translational initiation (as I found...

06 July 2014 5,304 1 View

How to support a claim that for gene A the relative levels of transcript reflect the transcriptional activity?

Transcript levels are frequently taken as a measure of gene activity, but other factors such as transcript stability also affect the levels of transcript. What data would be sufficient to...

10 November 2013 7,854 8 View

Courses in Bioinformatics and NGS

Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...

07 August 2013 8,000 4 View

Normalization to average as an alternative to using reference gene.

Is it valid to normalize gene expression to the average between biological repeats? Could that approach be used for large data sets were most genes show some degree of variation or is there a...

10 November 2012 7,773 7 View

Recipe for SYBR.

Anybody has a good recipe for homemade SYBR green mix for real time PCR? To my understanding, one of the key factors is the taq enzyme because most enzymes are inhibited by the SYBR dye.

10 November 2012 7,102 1 View

Can anyone recommend a protocol for Sybr Green Master mix?

Does anyone have a good recipe for homemade Sybr Green Master mix? I have tried a couple, and to my understanding the brand/variant of the DNA polymerase is a major factor. Any experiences as to...

10 November 2012 4,440 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View