In my current study, I am using NMM as fluorescence material. Most of the previous study mentioned the excitation spectra of NMM is 399nm and the emission spectra is 610nm. Is it possible that I can excite NMM at 435nm and take the emission at 610nm?
What is the absorption spectra maxims of your MMM material, based on the absorption maxmia only you can able to fix excitation wavelengths for your fluorescence emission measurements.
This paper has absorbance spectra of N-methylmesoporphyrin IX. It absorbs at 435 nm, although much less than at the shorter wavelength, so you should be able to use 435 nm excitation, just with a loss of sensitivity. You will still be able to use 610 nm emission.
http://nar.oxfordjournals.org/content/40/12/5432.full
Thank you Dr. Adam B Shaprio for sharing the article and your valuable suggestion. It really helped me out in designing my project.
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