As per ATCC guidelines, there should be a 0.01 mg/ml of insulin in the medium. Please explain if there are any noticeable changes (phenotypic and genotypic) in the cell line?
I have seen some answers saying that we can use DMEM instead of RPMI (11.89mM Glucose). But I want to culture them in a low glucose medium (5mM Glucose) as per my experiments and we don't have low...
06 July 2015 2,370 3 View
THP1 monocytes, despite of them being a suspension culture are getting adhered (all the cells) to the dish during serum starvation (at least 45mins to 1hr in basal RPMI). They are adhering so...
05 June 2015 6,790 8 View
I have used CD133/1 antibody (Human) but didn't observe a detectable amount of the protein in MCF-7 (although I did it only once). There are two possibilities here. 1. There's a mismatch of the...
02 March 2014 677 0 View
Some people feel the tickling but some won't. Are there any genetic reasons for this? Also, are laughter and tickling linked to any single operon?
08 September 2013 7,870 1 View
What is the difference between normal primers and nested primers and why are nested specfically used in TAIL PCR?
04 May 2013 6,309 8 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
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05 August 2024 4,407 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View