Srinivas Allanki

6 Questions 9 Answers 0 Followers

Questions related from Srinivas Allanki

Can we culture THP1 in Minimum Essential Medium instead of the ATCC suggested RPMI?

I have seen some answers saying that we can use DMEM instead of RPMI (11.89mM Glucose). But I want to culture them in a low glucose medium (5mM Glucose) as per my experiments and we don't have low...

07 July 2015 2,353 3 View

Why are THP1 monocytes getting adhered to the dish during serum starvation?

THP1 monocytes, despite of them being a suspension culture are getting adhered (all the cells) to the dish during serum starvation (at least 45mins to 1hr in basal RPMI). They are adhering so...

06 June 2015 6,774 8 View

What are the effects of the presence of insulin in the culture medium of MCF-7?

As per ATCC guidelines, there should be a 0.01 mg/ml of insulin in the medium. Please explain if there are any noticeable changes (phenotypic and genotypic) in the cell line?

03 March 2014 7,395 0 View

Can anyone suggest an antibody that can detect CD133 in MCF-7 cell line?

I have used CD133/1 antibody (Human) but didn't observe a detectable amount of the protein in MCF-7 (although I did it only once). There are two possibilities here. 1. There's a mismatch of the...

03 March 2014 656 0 View

What kind of hormones and genes or operons are responsible for the laughter caused due to tickling (gargalesis)?

Some people feel the tickling but some won't. Are there any genetic reasons for this? Also, are laughter and tickling linked to any single operon?

09 September 2013 7,854 1 View

Why do we use nested primers in TAIL PCR?

What is the difference between normal primers and nested primers and why are nested specfically used in TAIL PCR?

05 May 2013 6,293 8 View